The idea of this project was to create a multifunctional three-part system in a cost efficient and scalable manner. In order to achieve this, we have chosen to a assemble functional nano-robot capable of a variety of tasks at the nano- and micro-scale. The technique of DNA origami was chosen as a linker between the components due to the numerous advantages it offers in scability, functionality and the precise assembly of nanostructures. Since each component of the nanorover has a functional aspect, we plan to devise appropriate experiments to model and detect the functionality for the different components:

• The motion of our nano-robot depends on the magnetic fields around the Dynabeads, the orientation of individual magnetic dipoles on the surface of the Dynabeads
• Concentration and size quantification can also be performed via Dynamic Light Scattering and UV/Vis spectroscopy
• Confirmation of DNA origami attachment can be confirmed and visualized using AFM, cryo-TEM, TSEM, etc. Furthermore, extent of the origami folding can also be adjudged via UV/Vis spectroscopy.
• The bursting of the liposomes can be measured by various techniques depending on their size: such as fluoresecence microscopy (STORM) for LUVs and wide-field microscopy for GUVs.

Dynabead

DNA origami

Functionalized Liposome